Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast

Biosci Biotechnol Biochem. 2004 Jan;68(1):79-84. doi: 10.1271/bbb.68.79.

Abstract

The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Benzo(a)pyrene / metabolism
  • Catalysis
  • Coumarins / metabolism
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism*
  • DNA, Complementary
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Protein Engineering / methods
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Shiitake Mushrooms / enzymology*
  • Shiitake Mushrooms / genetics
  • Substrate Specificity

Substances

  • Coumarins
  • DNA, Complementary
  • Recombinant Proteins
  • 7-ethoxycoumarin
  • Benzo(a)pyrene
  • Cytochrome P-450 Enzyme System