Abstract
Three ovine-derived cathelicidins, SMAP29, OaBac5mini, and OaBac7.5mini, were compared with respect to their antibacterial activities and interactions with membranes. SMAP29 was confirmed to be alpha-helical, broad spectrum, and able to disrupt both the outer and the cytoplasmic membranes at relatively low concentrations. In contrast, the two proline- and arginine-rich OaBac peptides had more-modest antibacterial activities, reduced levels of lipopolysaccharide binding, and a lesser ability to depolarize the cytoplasmic membrane, consistent with a cytoplasmic target.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Anti-Bacterial Agents / chemistry
-
Anti-Bacterial Agents / isolation & purification
-
Anti-Bacterial Agents / pharmacology*
-
Antimicrobial Cationic Peptides / chemistry
-
Antimicrobial Cationic Peptides / isolation & purification
-
Antimicrobial Cationic Peptides / pharmacology*
-
Bacteria / drug effects*
-
Blood Proteins / chemistry
-
Blood Proteins / pharmacology*
-
Cathelicidins
-
Cell Membrane / drug effects
-
Circular Dichroism
-
Escherichia coli / drug effects
-
Escherichia coli / metabolism
-
Lipopolysaccharides / metabolism
-
Microbial Sensitivity Tests
-
Molecular Sequence Data
-
Sheep / metabolism*
Substances
-
Anti-Bacterial Agents
-
Antimicrobial Cationic Peptides
-
Blood Proteins
-
Cathelicidins
-
Lipopolysaccharides
-
SMAP29 protein, Ovis aries