Inhibition of the subgenomic hepatitis C virus replicon in huh-7 cells by 2'-deoxy-2'-fluorocytidine

Antimicrob Agents Chemother. 2004 Feb;48(2):651-4. doi: 10.1128/AAC.48.2.651-654.2004.

Abstract

2'-Deoxy-2'-fluorocytidine (FdC) is a potent inhibitor of the hepatitis C virus RNA replicon in culture, and FdC-5'-triphosphate is an effective inhibitor of the NS5B polymerase. Dynamic profiling of cell growth in an antiviral assay showed that FdC caused cytostasis due to an S-phase arrest. These observations demonstrate that FdC treatment is affecting both a viral target and a cellular target.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Cattle
  • Cell Division / drug effects
  • Cell Line
  • Deoxycytidine / analogs & derivatives*
  • Deoxycytidine / pharmacology*
  • Diarrhea Viruses, Bovine Viral / genetics
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / pharmacology
  • Hepacivirus / drug effects*
  • Hepacivirus / genetics*
  • Humans
  • RNA, Viral / biosynthesis*
  • RNA, Viral / genetics*
  • Replicon / drug effects*
  • Replicon / genetics*
  • S Phase / drug effects
  • Viral Nonstructural Proteins / antagonists & inhibitors

Substances

  • Antiviral Agents
  • Enzyme Inhibitors
  • RNA, Viral
  • Viral Nonstructural Proteins
  • Deoxycytidine
  • NS-5 protein, hepatitis C virus
  • 2'-fluoro-2'-deoxycytidine