Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis

Chem Commun (Camb). 2004 Feb 7:(3):274-5. doi: 10.1039/b313155f. Epub 2004 Jan 5.

Abstract

A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • Enzyme Stability
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / metabolism*
  • Hydrogen-Ion Concentration
  • Molecular Structure
  • Mutation / genetics*
  • Temperature
  • Thioglycosides / biosynthesis*
  • Thioglycosides / chemistry

Substances

  • Thioglycosides
  • Glycoside Hydrolases