Abstract
The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated. By investigation of the activities of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in the soluble fraction of G. oxydans, and cloning and expression of genes in Escherichia coli, it was found that both G6PDH and 6PGDH have NAD/NADP dual coenzyme specificities. It was suggested that the pentose phosphate pathway is responsible for NADH regeneration in G. oxydans.
MeSH terms
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Cloning, Molecular
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Coenzymes / metabolism*
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Escherichia coli / genetics
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Gluconobacter oxydans / enzymology*
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Gluconobacter oxydans / genetics
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Glucosephosphate Dehydrogenase / genetics
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Glucosephosphate Dehydrogenase / metabolism*
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NAD / metabolism
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NADP / metabolism
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Oxidation-Reduction
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Pentose Phosphate Pathway / physiology*
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Phosphogluconate Dehydrogenase / genetics
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Phosphogluconate Dehydrogenase / metabolism*
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Substrate Specificity
Substances
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Coenzymes
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NAD
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NADP
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Phosphogluconate Dehydrogenase
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Glucosephosphate Dehydrogenase