Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins

J Lipid Res. 2004 Apr;45(4):773-5. doi: 10.1194/jlr.D300035-JLR200. Epub 2004 Jan 16.

Abstract

Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel. To help overcome this limitation, we recommend a modification

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel / methods*
  • Electrophoresis, Polyacrylamide Gel / standards
  • Gels / chemistry
  • Humans
  • Lipoproteins / blood
  • Lipoproteins / isolation & purification*
  • Lipoproteins, HDL / blood
  • Lipoproteins, HDL / isolation & purification
  • Lipoproteins, LDL / blood
  • Lipoproteins, LDL / isolation & purification
  • Methods
  • Sensitivity and Specificity
  • Sucrose

Substances

  • Gels
  • Lipoproteins
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Sucrose