Serodiagnosis of chronic and acute Chagas' disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries

J Clin Microbiol. 2004 Jan;42(1):449-52. doi: 10.1128/JCM.42.1.449-452.2004.

Abstract

An enzyme-linked immunosorbent assay to diagnose Chagas' disease by a serological test was performed with Trypanosoma cruzi recombinant antigens (JL8, MAP, and TcPo). High sensitivity (99.4%) and specificity (99.3%) were obtained when JL8 was combined with MAP (JM) and tested with 150 serum samples from chagasic and 142 nonchagasic individuals. Moreover, JM also diagnosed 84.2% of patients in the acute phase of T. cruzi infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Antibodies, Protozoan / blood*
  • Antigens, Protozoan / immunology*
  • Chagas Disease / diagnosis*
  • Chronic Disease
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin G / blood
  • Recombinant Proteins / immunology
  • Sensitivity and Specificity
  • Serologic Tests
  • Trypanosoma cruzi / immunology*

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Immunoglobulin G
  • Recombinant Proteins