Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes

Methods Enzymol. 2003:370:553-67. doi: 10.1016/S0076-6879(03)70047-0.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Biochemistry / methods*
  • DNA / chemistry
  • DNA-Directed RNA Polymerases / chemistry*
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / enzymology*
  • Kinetics
  • Models, Chemical
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Structure, Tertiary
  • Sequence Homology, Nucleic Acid
  • Sigma Factor / chemistry*
  • Sigma Factor / metabolism

Substances

  • Oligonucleotides
  • Sigma Factor
  • DNA
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases