Abstract
It has previously been suggested that volume-regulated anion channels (VRACs) and store-operated channels (SOCs) interact with each other according to their expected colocalization in the plasma membrane of LNCaP cells. In order to study interactions between these two channels, we used 2-aminoethoxydiphenyl borate (2-APB) as a regular SOC inhibitor. Surprisingly 2-APB reduced VRAC activity in a dose-dependent manner (IC(50)=122.8 microM), but not 2,2-diphenyltetrahydrofuran (a structural analog of 2-APB). This effect was also present in keratinocytes. We conclude that 2-APB is an inhibitor of the VRAC family, and is also a potent tool to study the SOC-VRAC interaction in LNCaP cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Boron Compounds / pharmacology*
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Calcium / metabolism
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Calcium Signaling / drug effects*
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Calcium Signaling / physiology
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Cell Line
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Cell Line, Tumor
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Chloride Channels / antagonists & inhibitors*
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Dose-Response Relationship, Drug
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Egtazic Acid / analogs & derivatives*
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Egtazic Acid / pharmacology
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Furans / chemistry
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Furans / pharmacology
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Humans
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Hypotonic Solutions / pharmacology
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Inhibitory Concentration 50
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Keratinocytes / drug effects
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Keratinocytes / metabolism
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Male
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Membrane Potentials / drug effects
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Patch-Clamp Techniques
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Prostatic Neoplasms / metabolism
Substances
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Boron Compounds
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Chloride Channels
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Furans
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Hypotonic Solutions
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tetrahydrofuran
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Egtazic Acid
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2-aminoethoxydiphenyl borate
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1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
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Calcium