Abstract
It is shown that NADH can be effectively oxidized by 1-methyl-3-nitropyridine in non-enzymatic and enzyme-mediated processes. Mechanistic issues of these reactions are discussed. These processes seem to contribute to the observed cytotoxicity of 1-methyl-3-nitropyridine. A key role of 1-methyl-3-nitropyridinyl radical formed in the enzyme-mediated processes is emphasized.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis
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Cell Line, Tumor
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Drug Screening Assays, Antitumor
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Free Radicals / metabolism
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Kinetics
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Leukemia L1210
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NAD / metabolism*
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NADH Dehydrogenase / metabolism
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Oxidants / metabolism
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Oxidants / pharmacology*
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Oxidation-Reduction
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Oxygen / metabolism
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Pyridinium Compounds / metabolism
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Pyridinium Compounds / pharmacology*
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Superoxide Dismutase / metabolism
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Xanthine Oxidase / metabolism
Substances
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1-methyl-3-nitropyridinium
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Free Radicals
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Oxidants
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Pyridinium Compounds
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NAD
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Superoxide Dismutase
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Xanthine Oxidase
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NADH Dehydrogenase
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Oxygen