Structural analysis of 2D-gel-separated glycoproteins from human cerebrospinal fluid by tandem high-resolution mass spectrometry

Kristina Håkansson; Mark R Emmett; Alan G Marshall; Pia Davidsson; Carol L Nilsson

doi:10.1021/pr034030n

Structural analysis of 2D-gel-separated glycoproteins from human cerebrospinal fluid by tandem high-resolution mass spectrometry

J Proteome Res. 2003 Nov-Dec;2(6):581-8. doi: 10.1021/pr034030n.

Authors

Kristina Håkansson¹, Mark R Emmett, Alan G Marshall, Pia Davidsson, Carol L Nilsson

Affiliation

¹ Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, Florida 32310-3706, USA.

PMID: 14692451
DOI: 10.1021/pr034030n

Abstract

The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Electrophoresis, Gel, Two-Dimensional*
Glycoproteins / cerebrospinal fluid*
Glycoproteins / chemistry*
Glycoproteins / metabolism
Humans
Mass Spectrometry / methods*
Molecular Sequence Data
Peptides / cerebrospinal fluid
Peptides / chemistry
Peptides / metabolism
Protein Conformation
Protein Isoforms / cerebrospinal fluid
Protein Isoforms / chemistry
Protein Isoforms / metabolism

Substances

Glycoproteins
Peptides
Protein Isoforms