Abstract
p51/p63, a member of the p53 gene family, is structurally conserved among a wide range of organisms, although the transactivator (TA) and N-terminally truncated (deltaN) isotype producing property seems to vary. Since p51/p63 is thought to play important roles in skin, limb, and craniofacial development in mammals, we examined Xenopus laevis larval and adult tissues for expression of p51/p63. Temporal analyses indicated enhanced transcription of the deltaN form of p51/p63 in premetamorphosis phase (at stage 44-48). p51/p63-positive cells in the inner layer of larval skin expanded to the suprabasal layers during the stratification. The epithelium of limb buds and the maxillofacial ectodermal tissues in tadpoles had a high level expression of p51/p63. The cloned deltaN-A/gamma type Xenopus p51/p63 exhibited a dominant-negative activity against the human TA-A/gamma isotype in a reporter assay. These results suggest that tissue-specific p51/p63-inducing mechanism and isotype-specific transcriptional regulator activities of p51/p63 are conserved between mammals and frogs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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DNA, Ribosomal / genetics
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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Evolution, Molecular
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Female
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Gene Expression
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Genes, Tumor Suppressor
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HeLa Cells
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Humans
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Larva / growth & development
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Larva / metabolism
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Larva / ultrastructure
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Male
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Phosphoproteins / chemistry
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Phosphoproteins / genetics*
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Phosphoproteins / metabolism*
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Promoter Regions, Genetic / genetics
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Protein Structure, Tertiary
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Tissue Distribution
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Trans-Activators / chemistry
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Trans-Activators / genetics*
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Trans-Activators / metabolism*
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Trans-Activators / pharmacology
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Transcription Factors
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Transcriptional Activation
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Transfection
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Tumor Suppressor Proteins
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Xenopus / growth & development
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Xenopus / metabolism*
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Xenopus Proteins*
Substances
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DNA, Ribosomal
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DNA-Binding Proteins
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Phosphoproteins
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RNA, Messenger
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TP63 protein, Xenopus
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TP63 protein, human
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Trans-Activators
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Transcription Factors
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Tumor Suppressor Proteins
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Xenopus Proteins