Notes on theory and experimental conditions behind two-photon excitation microscopy

Microsc Res Tech. 2004 Jan 1;63(1):12-7. doi: 10.1002/jemt.10425.

Abstract

This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.

Publication types

  • Historical Article

MeSH terms

  • DNA / ultrastructure*
  • History, 20th Century
  • Microscopy, Confocal / history
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence, Multiphoton / history
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Models, Theoretical
  • Photons*
  • Sensitivity and Specificity
  • User-Computer Interface

Substances

  • DNA