Homonuclear Hartmann-Hahn transfer with reduced relaxation losses by use of the MOCCA-XY16 multiple pulse sequence

J Magn Reson. 2004 Jan;166(1):39-46. doi: 10.1016/j.jmr.2003.09.013.

Abstract

Homonuclear Hartmann-Hahn transfer is one of the most important building blocks in modern high-resolution NMR. It constitutes a very efficient transfer element for the assignment of proteins, nucleic acids, and oligosaccharides. Nevertheless, in macromolecules exceeding approximately 10 kDa TOCSY-experiments can show decreasing sensitivity due to fast transverse relaxation processes that are active during the mixing periods. In this article we propose the MOCCA-XY16 multiple pulse sequence, originally developed for efficient TOCSY transfer through residual dipolar couplings, as a homonuclear Hartmann-Hahn sequence with improved relaxation properties. A theoretical analysis of the coherence transfer via scalar couplings and its relaxation behavior as well as experimental transfer curves for MOCCA-XY16 relative to the well-characterized DIPSI-2 multiple pulse sequence are given.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • Algorithms*
  • Animals
  • Artifacts*
  • Chickens
  • Egg Proteins / chemistry
  • Muramidase / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Oligopeptides / chemistry*
  • RNA / chemistry*
  • Scattering, Radiation
  • Signal Processing, Computer-Assisted*
  • Spin Labels

Substances

  • Egg Proteins
  • Oligopeptides
  • Spin Labels
  • RNA
  • Muramidase