Abstract
We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice.
MeSH terms
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Amino Acid Sequence
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Cells, Cultured
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Chitin / analogs & derivatives
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Chitin / pharmacology
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Diterpenes / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant
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Isomerases / genetics*
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Isomerases / metabolism
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Molecular Sequence Data
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Oligosaccharides / pharmacology*
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Oryza / enzymology
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Oryza / genetics*
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Oryza / metabolism
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Phytoalexins
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Plant Extracts / biosynthesis*
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Plant Leaves / enzymology
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Plant Leaves / genetics
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Plant Leaves / radiation effects
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sesquiterpenes
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Terpenes
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Ultraviolet Rays
Substances
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DNA, Complementary
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Diterpenes
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Oligosaccharides
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Plant Extracts
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Plant Proteins
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RNA, Messenger
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Sesquiterpenes
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Terpenes
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Chitin
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DTC1 protein, Oryza sativa
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Isomerases
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Phytoalexins