Abstract
Overexpression of plant Bax Inhibitor-1 (BI-1) was able to suppress Bax-mediated cell death in yeast and Arabidopsis. Here, we demonstrate that reactive oxygen species production induced by the ectopic expression of Bax was insensitive to the coexpression of AtBI-1. Similarly, H2O2- or salicylic acid-mediated cell death also was suppressed in tobacco BY-2 cells overexpressing AtBI-1. To define the functional domain of AtBI-1 as a cell death suppressor, a truncated series of the AtBI-1 protein was analyzed in yeast possessing a galactose-inducible mammalian Bax. The results showed that DeltaC-AtBI-1 (with the C-terminal 14 amino acids deleted) lost the ability to sustain cell growth. Furthermore, a mutant protein in which the C-terminal seven amino acid residues of AtBI-1 were replaced with others lacking a coiled-coil structure failed to inhibit cell death, suggesting that the C-terminal region is essential for the inhibition of cell death. We also noted that the C-terminal hydrophilic region was interchangeable between animal and plant Bax inhibitors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological / drug effects
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Adaptation, Physiological / genetics
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Amino Acid Sequence
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Apoptosis / drug effects*
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Apoptosis / genetics
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Line
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Hydrogen Peroxide / pharmacology*
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Ion Channels / antagonists & inhibitors
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Ion Channels / physiology
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Oxygen / metabolism
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Plants, Genetically Modified
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-bcl-2*
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reactive Oxygen Species / metabolism
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Salicylic Acid / pharmacology*
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Sequence Homology, Amino Acid
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bcl-2-Associated X Protein
Substances
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ATBI-1 protein, Arabidopsis
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Arabidopsis Proteins
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Ion Channels
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Membrane Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Reactive Oxygen Species
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bcl-2-Associated X Protein
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Hydrogen Peroxide
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Salicylic Acid
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Oxygen