Aim: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.
Methods: Hsp60 DNA was amplified by PCR and inserted into the prokaryote expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal experiments.
Results: DNA sequence analysis showed Hsp60 DNA was the same as GenBank's research. Hsp60 recombinant protein accounted for 27.2% of the total bacterial protein, and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 itself.
Conclusion: Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection.