Antifungal clerodane diterpenes from Macaranga monandra (L) Muell. et Arg. (Euphorbiaceae)

Martin A Salah; Erdal Bedir; Ngeh J Toyang; Ikhlas A Khan; M Dewayne Harries; David E Wedge

doi:10.1021/jf034682w

Antifungal clerodane diterpenes from Macaranga monandra (L) Muell. et Arg. (Euphorbiaceae)

J Agric Food Chem. 2003 Dec 17;51(26):7607-10. doi: 10.1021/jf034682w.

Authors

Martin A Salah¹, Erdal Bedir, Ngeh J Toyang, Ikhlas A Khan, M Dewayne Harries, David E Wedge

Affiliation

¹ National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA.

PMID: 14664515
DOI: 10.1021/jf034682w

Abstract

Hexane and ethyl acetate phases of the methanol extract of Macaranga monandra showed fungal growth inhibition of Colletotrichum acutatum, C. fragariae and C. gloeosporioides, Fusarium oxysporum, Botrytis cinerea, Phomopsis obscurans, and P. viticola. Bioassay-guided fractionation led to the isolation of two active clerodane-type diterpenes that were elucidated by spectroscopic methods (1D-, 2D-NMR, and MS) as kolavenic acid and 2-oxo-kolavenic acid. A 96-well microbioassay revealed that kolavenic acid and 2-oxo-kolavenic acid produced moderate growth inhibition in Phomopsis viticola and Botrytis cinerea.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetates
Ascomycota / drug effects
Botrytis / drug effects
Colletotrichum / drug effects
Diterpenes, Clerodane / pharmacology*
Euphorbiaceae / chemistry*
Fungicides, Industrial / pharmacology*
Fusarium / drug effects
Hexanes
Plant Extracts / chemistry*

Substances

Acetates
Diterpenes, Clerodane
Fungicides, Industrial
Hexanes
Plant Extracts
ethyl acetate