Application of genome-wide expression analysis to identify molecular markers useful in monitoring industrial fermentations

Vincent J Higgins; Peter J Rogers; Ian W Dawes

doi:10.1128/AEM.69.12.7535-7540.2003

Application of genome-wide expression analysis to identify molecular markers useful in monitoring industrial fermentations

Appl Environ Microbiol. 2003 Dec;69(12):7535-40. doi: 10.1128/AEM.69.12.7535-7540.2003.

Authors

Vincent J Higgins¹, Peter J Rogers, Ian W Dawes

Affiliation

¹ Clive and Vera Ramaciotti Centre for Gene Function Analysis, University of New South Wales, Sydney, New South Wales 2052, Australia. v.higgins@unsw.edu.au

Abstract

Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae identified the YOR387c and YGL258w homologues as highly inducible in zinc-depleted conditions. Induction was specific for zinc deficiency and was dependent on Zap1p. The results indicate that these sequences may be valuable molecular markers for detecting zinc deficiency in industrial fermentations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Culture Media
Fermentation
Gene Expression Profiling*
Gene Expression Regulation, Fungal
Genetic Markers*
Genome, Fungal*
Industrial Microbiology / methods
Oligonucleotide Array Sequence Analysis*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / metabolism*
Zinc / metabolism

Substances

Culture Media
Genetic Markers
Saccharomyces cerevisiae Proteins
Zinc