Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry

Scott C Dorman; John P Harrington; Melinda S Martin; Timothy V Johnson

doi:10.1016/j.jinorgbio.2003.10.004

Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry

J Inorg Biochem. 2004 Jan;98(1):185-8. doi: 10.1016/j.jinorgbio.2003.10.004.

Authors

Scott C Dorman¹, John P Harrington, Melinda S Martin, Timothy V Johnson

Affiliation

¹ Department of Chemistry, Birmingham Southern College, Birmingham, AL 35254, USA. sdorman@bsc.edu

PMID: 14659648
DOI: 10.1016/j.jinorgbio.2003.10.004

Abstract

The formal reduction potential (Eo') of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (+/-0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant.

MeSH terms

Animals
Electrochemistry / methods
Hemoglobins / chemistry*
Horses
Myoglobin / chemistry
Oligochaeta / chemistry*
Oxidation-Reduction
Reproducibility of Results
Spectrophotometry / methods

Substances

Hemoglobins
Myoglobin