Analysis of the RNA helicase A gene in human lung cancer

Oncol Rep. 2004 Jan;11(1):253-8.

Abstract

In this study we investigated the RNA helicase A (RHA) gene localized on chromosome 1q25 in 15 lung cancer cell lines, 54 primary carcinomas, bronchial epithelial cells and normal lung. Both RT-PCR and real-time RT-PCR showed that RHA is over-expressed in tumour samples compared to normal lung tissues (p=0.01). There was a tendency for higher expression levels in small cell lung cancer compared to non-small cell carcinomas. Over-expression of Y-162 correlated with high grade tumours (p=0.023). However, there was no correlation with tumour stage and survival. Our observations are compatible with the role of RHA in lung cancer development.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / genetics*
  • Blotting, Northern
  • Cell Line
  • Cell Line, Tumor
  • DEAD-box RNA Helicases
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology*
  • Neoplasm Proteins
  • Nucleic Acid Hybridization / methods
  • RNA Helicases / genetics*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Autoantigens
  • Neoplasm Proteins
  • RNA, Neoplasm
  • DHX9 protein, human
  • DEAD-box RNA Helicases
  • RNA Helicases