[TLR4 mRNA expression and liver injury in LPS-induced mouse]

Hunan Yi Ke Da Xue Xue Bao. 2003 Jun;28(3):217-20.
[Article in Chinese]

Abstract

Objective: To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).

Methods: Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.

Results: A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.

Conclusion: The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.

MeSH terms

  • Animals
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / metabolism*
  • Chemical and Drug Induced Liver Injury / pathology
  • Endotoxemia / chemically induced
  • Endotoxemia / metabolism*
  • Lipopolysaccharides
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / genetics
  • Mice
  • Mice, Inbred BALB C
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Signal Transduction
  • Toll-Like Receptor 4
  • Toll-Like Receptors

Substances

  • Lipopolysaccharides
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Toll-Like Receptor 4
  • Toll-Like Receptors