In order to investigate the effect of vitamins-minerals supplementation on the nutritional status of Chinese women, 252 villagers aged 20-50 years old were selected as the participants in the study in Longli County, Guizhou Province. Within Twenty two weeks, half of the participants received the supplements and half had placebo. The results showed that in comparison with the control, the women with supplementation represented a higher urine excretion of vitamin B1 and B2 after a loading dosage and, a higher concentration of serum iron and zinc and a lower prevalence of anemia. They kept their facial moisture and grease better than those of the controls. They also felt better in sleeping, having appetite, and doing physical activities. The results indicated that the supplementation of vitamins-minerals might improve the nutritional status of rural Chinese women.