A questionnaire was used for investigation of 788 workers from three transport enterprises. The questionnaire was used for evaluation of occupational environment, lifestyle, psychosocial factors and self-evaluation of health. The most harmful occupational factors are unsatisfactory microclimatic conditions, diesel fuel, cooling oil, vibration, musculoskeletal load, and mental tension. Worker's lifestyle isn't healthy: 46% of them are smoking, 83% are drinking alcohol, 53% are physically inactive, 82% have bad nutrition regimen, 27% are everyday suffering from stressful situations. Main health impairments are musculoskeletal (46.2%), respiratory tract (22.2%), gastrointestinal (17.3%) and central nervous system (32.2%) diseases. Musculoskeletal disorders are statistically related to bad ergonomic work conditions, long workday hours, aged employees and long work experience of workers (p<0.05-0.002).