Abstract
In this paper, we demonstrate how one can chemically design Raman dye-functionalized nanoparticle probes with specific protein-binding affinities and use these probes, coupled with surface-enhanced Raman scattering (SERS) spectroscopy, to perform multiplexed screening of protein-small molecule interactions and protein-protein interactions in a protein microarray format.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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2,4-Dinitrophenol / chemistry
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Antibodies, Monoclonal / chemistry
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Biotin / chemistry
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Digoxigenin / chemistry
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Hydroquinones / chemistry
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Nanotechnology
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Particle Size
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Protein Array Analysis / methods*
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Proteins / analysis*
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Silver / chemistry
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Spectrum Analysis, Raman / methods*
Substances
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Antibodies, Monoclonal
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Hydroquinones
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Proteins
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Silver
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Biotin
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Digoxigenin
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2,4-Dinitrophenol
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hydroquinone