Raman dye-labeled nanoparticle probes for proteins

J Am Chem Soc. 2003 Dec 3;125(48):14676-7. doi: 10.1021/ja0366235.

Abstract

In this paper, we demonstrate how one can chemically design Raman dye-functionalized nanoparticle probes with specific protein-binding affinities and use these probes, coupled with surface-enhanced Raman scattering (SERS) spectroscopy, to perform multiplexed screening of protein-small molecule interactions and protein-protein interactions in a protein microarray format.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 2,4-Dinitrophenol / chemistry
  • Antibodies, Monoclonal / chemistry
  • Biotin / chemistry
  • Digoxigenin / chemistry
  • Hydroquinones / chemistry
  • Nanotechnology
  • Particle Size
  • Protein Array Analysis / methods*
  • Proteins / analysis*
  • Silver / chemistry
  • Spectrum Analysis, Raman / methods*

Substances

  • Antibodies, Monoclonal
  • Hydroquinones
  • Proteins
  • Silver
  • Biotin
  • Digoxigenin
  • 2,4-Dinitrophenol
  • hydroquinone