3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected types of meat, seafood, and poultry: collaborative study

J AOAC Int. 2003 Sep-Oct;86(5):947-53.

Abstract

The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Four foods--cooked, diced chicken; cured ham; smoked salmon; and pepperoni--were analyzed for S. aureus by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 4 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Chickens
  • Colony Count, Microbial / instrumentation
  • Colony Count, Microbial / methods*
  • Food Handling
  • Meat / microbiology*
  • Meat Products / microbiology
  • Poultry / microbiology*
  • Reproducibility of Results
  • Salmon / microbiology
  • Seafood / microbiology*
  • Staphylococcus aureus / isolation & purification*
  • Swine