Alternative NMR method for quantitative determination of acyl positional distribution in triacylglycerols and related compounds

S Simova; G Ivanova; S L Spassov

doi:10.1016/j.chemphyslip.2003.08.003

Alternative NMR method for quantitative determination of acyl positional distribution in triacylglycerols and related compounds

Chem Phys Lipids. 2003 Dec;126(2):167-76. doi: 10.1016/j.chemphyslip.2003.08.003.

Authors

S Simova¹, G Ivanova, S L Spassov

Affiliation

¹ Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. sds@orgchm.bas.bg

PMID: 14623452
DOI: 10.1016/j.chemphyslip.2003.08.003

Abstract

High-resolution 13C NMR spectroscopy has been used to analyze the positional distribution of fatty acids in model triacylglycerols. A novel method for quantitative determination of the positional distribution of unsaturated chains in triacylglycerols simultaneously with the ratio of saturated/unsaturated acyl chains has been proposed, utilizing the chemical shift differences of the aliphatic atoms C4, C5, and C6. The use of HSQC-TOCSY spectra allows unequivocal proof of the position of the unsaturated chain as well as complete assignment of the 13C NMR signals in tripalmitin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acylation
Carbon Isotopes
Fatty Acids / chemistry
Magnetic Resonance Spectroscopy
Molecular Structure
Stereoisomerism
Triglycerides / chemistry*

Substances

Carbon Isotopes
Fatty Acids
Triglycerides
tripalmitin