Abstract
The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of gamma-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acclimatization
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Buthionine Sulfoximine / pharmacology
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Enzyme Inhibitors / pharmacology
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Glutamate-Cysteine Ligase / antagonists & inhibitors
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Glutamate-Cysteine Ligase / drug effects
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Glutamate-Cysteine Ligase / metabolism
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Glutathione / biosynthesis*
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Glutathione / chemistry
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Glutathione Disulfide / analysis
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Glutathione Disulfide / biosynthesis*
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Glutathione Disulfide / chemistry
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Glutathione Peroxidase / drug effects
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Glutathione Peroxidase / metabolism
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Glutathione Reductase / drug effects
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Glutathione Reductase / metabolism
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Glutathione Transferase / drug effects
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Glutathione Transferase / metabolism
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Hydrogen Peroxide / analysis
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Malondialdehyde / analysis
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Oxidation-Reduction
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Oxidative Stress / drug effects
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Oxidative Stress / physiology
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Plant Leaves / chemistry
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Plant Leaves / drug effects
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Plant Leaves / metabolism
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Plant Roots / drug effects
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Plant Roots / metabolism
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Sodium Chloride / pharmacology*
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Solanum lycopersicum / drug effects
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Solanum lycopersicum / enzymology
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Solanum lycopersicum / metabolism*
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Species Specificity
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Up-Regulation
Substances
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Enzyme Inhibitors
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Sodium Chloride
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Malondialdehyde
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Buthionine Sulfoximine
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Hydrogen Peroxide
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Glutathione Peroxidase
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Glutathione Reductase
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Glutathione Transferase
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Glutamate-Cysteine Ligase
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Glutathione
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Glutathione Disulfide