Induction of tetraploid gynogenesis in the European sea bass (Dicentrarchus labrax L.)

Genetica. 2003 Oct;119(2):225-8. doi: 10.1023/a:1026077405294.

Abstract

A preliminary study on tetraploid gynogenetic induction in the European sea bass was performed by pressure-blocking the second polar body release and the first cleavage in eggs fertilized with ultraviolet-irradiated sperm. Fertilization of eggs with genetically inactivated sperm produced only haploid development that terminated around hatching. Pressure treatments (8.500 psi for 2 min) applied at 6 and 65 min after fertilization (a.f.) produced variable levels (7-95%) of tetraploid larvae at hatching. A small proportion of mosaics (3.8n/4.2n) was also recorded.

MeSH terms

  • Animals
  • Bass / embryology
  • Bass / genetics*
  • Cell Count
  • Diploidy
  • Female
  • Flow Cytometry
  • Heat-Shock Response
  • Male
  • Polyploidy*
  • Sperm-Ovum Interactions*
  • Spermatozoa / physiology
  • Spermatozoa / radiation effects
  • Ultraviolet Rays
  • Zygote / physiology
  • Zygote / radiation effects