Hydroxamic acids are found as chelates in plants, as metabolites of bacteria and funges. Some aerobic microorganisms synthesize hydroxamic acids, which transmit iron from environment to the cells of the plants. Fourteen hydroxamic acids were synthesized and their interaction with iron (II and III) was investigated. The purpose of this investigation was synthesis of hydroxamic acids, creation of stable iron chelate with one of these acids, preparation of the methods of analysis, investigation of the stability of chelate and application for prophylaxis and treatment of iron deficiency anemia. We used octanhydroxamic acid for creation of chelate with iron. The synthesis of this acid was the cheapest and compound with iron appeared stable. Iron chelates after acid hydrolysis were analyzed by quantity of iron (9.8-12.3%). We used complexometric titration method with dinatrium salt of ethylendiamintetraacetic acid without indication for quantitative analysis. Iron (II) chelate is less stable, therefore, we used Fe (III) octanhydroxamate for further investigations. Preliminary trials with rabbits confirm rapid assimilation of iron (III) chelate from gastrointestinal tract and activation of erythropoiesis.