Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Mary E Kauffman; William K Keener; Scott R Clingenpeel; Mary E Watwood; David W Reed; Yoshiko Fujita; R Michael Lehman

doi:10.1016/j.mimet.2003.07.001

Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

J Microbiol Methods. 2003 Dec;55(3):801-5. doi: 10.1016/j.mimet.2003.07.001.

Authors

Mary E Kauffman¹, William K Keener, Scott R Clingenpeel, Mary E Watwood, David W Reed, Yoshiko Fujita, R Michael Lehman

Affiliation

¹ Idaho National Engineering and Environmental Laboratory, PO Box 1625, MS-2203, Idaho Falls, ID 83415, USA.

PMID: 14607424
DOI: 10.1016/j.mimet.2003.07.001

Abstract

3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acetylene / analogs & derivatives*
Acetylene / metabolism
Burkholderia cepacia / enzymology
Burkholderia cepacia / metabolism*
Catechols / metabolism*
Fluorescent Dyes / metabolism*
Microscopy, Fluorescence
Pseudomonas / enzymology
Pseudomonas / metabolism*
Ralstonia / enzymology
Ralstonia / metabolism*
Toluene / metabolism*

Substances

Catechols
Fluorescent Dyes
3-methylcatechol
Toluene
Acetylene