Restriction enzyme analysis of ribosomal DNA shows that Candida inconspicua clinical isolates can be misidentified as Candida norvegensis with traditional diagnostic procedures

J Clin Microbiol. 2003 Nov;41(11):5250-3. doi: 10.1128/JCM.41.11.5250-5253.2003.

Abstract

We identified 29 yeast isolates from 22 patients using the API ID32C panel. Twenty-eight of these isolates were Candida norvegensis and one was C. inconspicua. Although C. norvegensis is considered a pseudohypha-producing species, only one isolate produced pseudohyphae. Restriction enzyme analysis of PCR-amplified ribosomal DNA with four different enzymes proved that all isolates were C. inconspicua.

MeSH terms

  • Candida / classification*
  • Candida / genetics*
  • Candida / isolation & purification
  • Candidiasis / classification
  • Candidiasis / diagnosis*
  • DNA, Ribosomal / genetics*
  • Humans
  • Inpatients
  • Outpatients
  • Polymorphism, Restriction Fragment Length
  • Reproducibility of Results
  • Restriction Mapping / methods

Substances

  • DNA, Ribosomal