Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids

Thorsten Kaiser; Alena Hermann; Jan T Kielstein; Stefan Wittke; Sebastian Bartel; Ronald Krebs; Frank Hausadel; Meike Hillmann; Igor Golovko; Peer Koester; Hermann Haller; Eva M Weissinger; Danilo Fliser; Harald Mischak

doi:10.1016/s0021-9673(03)00712-x

Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids

J Chromatogr A. 2003 Sep 26;1013(1-2):157-71. doi: 10.1016/s0021-9673(03)00712-x.

Authors

Thorsten Kaiser¹, Alena Hermann, Jan T Kielstein, Stefan Wittke, Sebastian Bartel, Ronald Krebs, Frank Hausadel, Meike Hillmann, Igor Golovko, Peer Koester, Hermann Haller, Eva M Weissinger, Danilo Fliser, Harald Mischak

Affiliation

¹ Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.

PMID: 14604117
DOI: 10.1016/s0021-9673(03)00712-x

Abstract

Combination of capillary electrophoresis with mass spectrometry (CE-MS) allows generation of polypeptide patterns of body fluids. In a single CE-MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (M(r) > 10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected. Comparison to the normal urine pattern yielded a surprisingly low consensus: a number of polypeptides present in urine were missing. We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dialysis
Electrophoresis, Capillary / methods*
Peptides / analysis*
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Peptides