Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000

C Guilhot; B Gicquel; C Martín

doi:10.1016/0378-1097(92)90152-e

Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000

FEMS Microbiol Lett. 1992 Nov 1;77(1-3):181-6. doi: 10.1016/0378-1097(92)90152-e.

Authors

C Guilhot¹, B Gicquel, C Martín

Affiliation

¹ Unité de Génie Microbiologique, URA1300 du CNRS, Institut Pasteur, Paris, France.

PMID: 1459406
DOI: 10.1016/0378-1097(92)90152-e

Abstract

Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30 degrees C but not at 39 degrees C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / genetics
Genes, Bacterial
Genetic Vectors
Mutagenesis
Mycobacterium / genetics*
Plasmids*
Replicon
Temperature
Transformation, Genetic