[Detection of the DeltaF508 trinucleotide deletion in the human CFTR gene by UV immobilization on nylon of a complex of a PCR fragment with a highly specific probe]

M R Kabilov; D V Pyshnyĭ; G M Dymshits; V F Zarytova; E M Ivanova

[Detection of the DeltaF508 trinucleotide deletion in the human CFTR gene by UV immobilization on nylon of a complex of a PCR fragment with a highly specific probe]

Mol Biol (Mosk). 2003 Sep-Oct;37(5):793-800.

[Article in Russian]

Authors

M R Kabilov¹, D V Pyshnyĭ, G M Dymshits, V F Zarytova, E M Ivanova

Affiliation

¹ Novosiberian Institute of Bioorganic Chemistry, Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.

PMID: 14593915

Abstract

Deletion delta F508 has been revealed in PCR-amplified regions of human gene CFTR by color detection of the hybridization complex obtained by ligation of a tandem of short oligonucleotides on a DNA template followed by UV immobilization on nylon. The method allows reliable detection of the three-nucleotide deletion (insertion). The nonspecific signal depends on the nucleotide composition of the biotinylated tandem component. A significant level of the specific signal was achieved by using the PCR-amplified DNA fragments of different length (200-400 bp) irrespective of the position of the tandem-binding site in their sequences.

Publication types

English Abstract

MeSH terms

Base Sequence
Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
DNA Primers
DNA Probes*
Humans
Nucleic Acid Hybridization
Nylons
Sequence Deletion*
Ultraviolet Rays

Substances

CFTR protein, human
DNA Primers
DNA Probes
Nylons
Cystic Fibrosis Transmembrane Conductance Regulator