Abstract
Embryonic chick ciliary ganglion (CG) neurons obtained from E7-E8 ganglia maintained in serum-free medium were stimulated with 50 microM muscarine. A fast hyperpolarization of the membrane potential was observed in 25% of the cells tested, that in some cases was associated with a slower depolarization. Accordingly, in voltage clamp experiments, either an outward current or a biphasic current response could be observed. Single-channel experiments provide evidence that these signals can be associated to the activation of a K(+) channel whose conductance is 20 pS.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chick Embryo
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Ganglia, Parasympathetic / drug effects
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Ganglia, Parasympathetic / physiology*
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Muscarine / pharmacology
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Muscarinic Agonists / pharmacology
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Neurons / drug effects
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Neurons / physiology*
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Patch-Clamp Techniques
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Potassium Channels / drug effects
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Potassium Channels / physiology*
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Receptors, Cholinergic / drug effects
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Receptors, Cholinergic / physiology*
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Receptors, Muscarinic / drug effects
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Receptors, Muscarinic / physiology*
Substances
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Muscarinic Agonists
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Potassium Channels
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Receptors, Cholinergic
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Receptors, Muscarinic
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Muscarine