Isolation and characterization of new epothilone analogues from recombinant Myxococcus xanthus fermentations

Courtney M Starks; Yiqing Zhou; Fenghua Liu; Peter J Licari

doi:10.1021/np030218+

Isolation and characterization of new epothilone analogues from recombinant Myxococcus xanthus fermentations

J Nat Prod. 2003 Oct;66(10):1313-7. doi: 10.1021/np030218+.

Authors

Courtney M Starks¹, Yiqing Zhou, Fenghua Liu, Peter J Licari

Affiliation

¹ Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA.

PMID: 14575429
DOI: 10.1021/np030218+

Abstract

Nine new epothilone analogues (6-8, 10, 11a, 11b, 12, 13, and 15) were isolated from fermentations of Myxococcus xanthus strains engineered with modified polyketide synthase genes. The epothilone structures were elucidated primarily through interpretation of 1D and 2D NMR data. 4-Desmethyl-10,11-didehydroepothilone D (6) displayed activity against several tumor cell lines, including a multi-drug-resistant cell line.

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / isolation & purification*
Antineoplastic Agents / pharmacology
Cell Line, Tumor / drug effects
Drug Resistance, Multiple / drug effects
Drug Screening Assays, Antitumor
Epothilones / chemistry
Epothilones / isolation & purification*
Epothilones / pharmacology
Fermentation
Humans
Inhibitory Concentration 50
Molecular Structure
Myxococcus xanthus / chemistry*
Nuclear Magnetic Resonance, Biomolecular

Substances

Antineoplastic Agents
Epothilones