Low-pathogenic avian influenza virus (AV) continues to be isolated from the live bird markets (LBMs) in the Northeasten United States. Recent years have seen increasing numbers of these markets opening and an expansion of the type of animals they sell in conjunction with traditional live poultry. Specific-pathogen-free chickens were released into the livestock area of 13 New York City LBMs and then tested for evidence of AIV. We were able to recover virus or demonstrate seroconversion among the chickens introduced to four of the markets.