ELISA test for the detection of influenza H7 antibodies in avian sera

G Sala; P Cordioli; A Moreno-Martin; M Tollis; E Brocchi; A Piccirillo; A Lavazza

doi:10.1637/0005-2086-47.s3.1057

ELISA test for the detection of influenza H7 antibodies in avian sera

Avian Dis. 2003;47(3 Suppl):1057-9. doi: 10.1637/0005-2086-47.s3.1057.

Authors

G Sala¹, P Cordioli, A Moreno-Martin, M Tollis, E Brocchi, A Piccirillo, A Lavazza

Affiliation

¹ Istituto Zooprofilattico della Lombardia ed Emilia Romagna B.Ubertini Via A. Bianchi, 9-25124 Brescia, Italy.

PMID: 14575110
DOI: 10.1637/0005-2086-47.s3.1057

Abstract

Using a monoclonal antibody (MAb) specific for the H7 influenza surface glycoproteins, a serological enzyme-linked immunosorbent assay (ELISA) test has been developed. This MAb was made using the low-pathogenicity (LP) avian influenza (AI) strain (BS2676/99) isolated in Italy during a recent outbreak. The test is able to detect H7 antibodies in avian sera. The H7 ELISA has a 99% concordance of results with the classical hemagglutination inhibition (HI) test.

MeSH terms

Allantois / immunology
Animals
Antibodies, Monoclonal
Antibodies, Viral / blood*
Antibody Specificity
Enzyme-Linked Immunosorbent Assay / methods*
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / immunology
Influenza A virus / immunology*
Italy
Poultry
Reproducibility of Results
Turkeys

Substances

Antibodies, Monoclonal
Antibodies, Viral
Hemagglutinin Glycoproteins, Influenza Virus