Abstract
A complementary DNA encoding human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned and introduced into tomato (Lycopersicon esculentum cv. Seokwang) using Agrobacterium-mediated transformation. Genomic PCR and Northern blot analysis demonstrated the integration of the construction into the plant nuclear genome and expression of the hGM-CSF in transgenic tomato. The cell suspension culture was established from leaf-derived calli of the transgenic tomato plants transformed with the hGM-CSF gene. Recombinant hGM-CSF was synthesized by the transgenic cell culture and secreted into the growth medium at 45 microg l(-1) after 10 d' cultivation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
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Humans
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Plant Leaves / genetics
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Plant Leaves / growth & development
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Plant Leaves / metabolism
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Plants, Genetically Modified / metabolism
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Protein Engineering / methods*
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Recombinant Proteins / biosynthesis
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Solanum lycopersicum / genetics*
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Solanum lycopersicum / growth & development
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Solanum lycopersicum / metabolism*
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Tissue Distribution
Substances
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Recombinant Proteins
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Granulocyte-Macrophage Colony-Stimulating Factor