Hydrogen bonds lengths in nucleic acids estimated from longitudinal nitrogen-15 relaxation

Dimitri Bytchenkoff; Geoffrey Bodenhausen

doi:10.1016/s1090-7807(03)00271-4

Hydrogen bonds lengths in nucleic acids estimated from longitudinal nitrogen-15 relaxation

J Magn Reson. 2003 Nov;165(1):1-8. doi: 10.1016/s1090-7807(03)00271-4.

Authors

Dimitri Bytchenkoff¹, Geoffrey Bodenhausen

Affiliation

¹ Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland.

PMID: 14568512
DOI: 10.1016/s1090-7807(03)00271-4

Abstract

A new NMR method has been designed for the measurement of the longitudinal relaxation rates of both donor and acceptor nitrogen-15 nuclei in Watson-Crick base pairs in 15N-enriched nucleic acids. The experiment was applied to a 22-nucleotide RNA hairpin. The lengths of four hydrogen bonds could be estimated from the longitudinal relaxation rates.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Algorithms*
Base Pairing*
Crystallography / methods*
Hydrogen / chemistry*
Hydrogen Bonding
Magnetic Resonance Spectroscopy / methods*
Models, Chemical
Nitrogen Isotopes
Nucleic Acid Conformation*
Nucleic Acids / chemistry
Polynucleotides / chemistry
RNA / chemistry*

Substances

Nitrogen Isotopes
Nucleic Acids
Polynucleotides
RNA
Hydrogen