Cell surface oligonucleotide-binding proteins of human squamous carcinoma A431 cells

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1715-9. doi: 10.1081/NCN-120023121.

Abstract

Affinity modified with Flu-DAP-p(N)16degU oligonucleotide-binding proteins were isolated by affinity chromatography using Ultrogel A2-anti fluorescein antibodies. After separation by SDS-PAGE the proteins with molecular masses about 68 kDa were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e and albumin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Carcinoma, Squamous Cell
  • Chromatography, Affinity
  • Chromatography, Gel
  • Humans
  • Keratin-10
  • Keratin-2
  • Keratins / chemistry
  • Keratins / isolation & purification
  • Keratins / metabolism
  • Membrane Proteins / chemistry
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Molecular Weight
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / isolation & purification
  • Neoplasm Proteins / metabolism
  • Oligonucleotides / metabolism*
  • Protein Binding
  • Serum Albumin / isolation & purification
  • Serum Albumin / metabolism
  • Tumor Cells, Cultured

Substances

  • KRT10 protein, human
  • KRT2 protein, human
  • Keratin-2
  • Membrane Proteins
  • Neoplasm Proteins
  • Oligonucleotides
  • Serum Albumin
  • Keratin-10
  • Keratins