Binding studies of eukaryotic initiation factor eIF4E with novel mRNA dinucleotide cap analogues

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1703-6. doi: 10.1081/NCN-120023118.

Abstract

Studies on the interaction of the murine translation initiation factor 4E with two new-synthesized cap-analogues, modified at C2' of 7-methylguanosine, have been performed by means of the fluorescence titration method. No difference in the binding affinity for eIF4E was observed compared with the "anti reversed" cap analogues, possessing the analogous modifications at C3'. Potential significance of the novel caps as research tools for examination of the nuclear cap binding complex CBC80/20 has been discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Dinucleoside Phosphates
  • Eukaryotic Initiation Factor-4E / metabolism*
  • Kinetics
  • Mice
  • Protein Binding
  • Protein Biosynthesis
  • RNA Caps / chemistry
  • RNA Caps / metabolism*
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism*
  • Structure-Activity Relationship

Substances

  • Dinucleoside Phosphates
  • Eukaryotic Initiation Factor-4E
  • RNA Caps
  • RNA, Messenger
  • 7-methyl-diguanosine triphosphate