New cell-cell adhesion inhibitors from Streptomyces sp. UMA-044

J Antibiot (Tokyo). 2003 Aug;56(8):673-81. doi: 10.7164/antibiotics.56.673.

Abstract

Three compounds, NP25301 (1), NP25302 (2) and bohemamine (3), inhibitors of cell adhesion based on LFA-1/ICAM-1, were isolated from the cultured broth of the strain Streptomyces sp. UMA-044. New compounds 1 and 2 were identified as 2-(3'-carbamoylphenoxy)acrylic acid methyl ester and deoxybohemamine, respectively, based on spectroscopic analyses. Compounds 1 approximately 3 inhibited adhesion of HL-60 cells to CHO-ICAM-1 cells at IC50 values of 29.5 microg/ml for 1, 24.3 microg/ml for 2, and 27.2 microg/ml for 3.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Benzamides / chemistry
  • Benzamides / isolation & purification
  • Benzamides / pharmacology
  • CHO Cells
  • Cell Adhesion / drug effects
  • Cell Aggregation / drug effects*
  • Cinnamates / chemistry
  • Cinnamates / isolation & purification
  • Cinnamates / pharmacology
  • Cricetinae
  • Fermentation
  • HL-60 Cells / drug effects
  • HL-60 Cells / metabolism
  • Humans
  • Intercellular Adhesion Molecule-1 / drug effects*
  • Pyrrolizidine Alkaloids* / chemistry
  • Pyrrolizidine Alkaloids* / isolation & purification
  • Pyrrolizidine Alkaloids* / pharmacology
  • Streptomyces

Substances

  • Benzamides
  • Cinnamates
  • NP 25301
  • NP 25302
  • Pyrrolizidine Alkaloids
  • Intercellular Adhesion Molecule-1
  • bohemamine