Abstract
Three compounds, NP25301 (1), NP25302 (2) and bohemamine (3), inhibitors of cell adhesion based on LFA-1/ICAM-1, were isolated from the cultured broth of the strain Streptomyces sp. UMA-044. New compounds 1 and 2 were identified as 2-(3'-carbamoylphenoxy)acrylic acid methyl ester and deoxybohemamine, respectively, based on spectroscopic analyses. Compounds 1 approximately 3 inhibited adhesion of HL-60 cells to CHO-ICAM-1 cells at IC50 values of 29.5 microg/ml for 1, 24.3 microg/ml for 2, and 27.2 microg/ml for 3.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Benzamides / chemistry
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Benzamides / isolation & purification
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Benzamides / pharmacology
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CHO Cells
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Cell Adhesion / drug effects
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Cell Aggregation / drug effects*
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Cinnamates / chemistry
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Cinnamates / isolation & purification
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Cinnamates / pharmacology
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Cricetinae
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Fermentation
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HL-60 Cells / drug effects
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HL-60 Cells / metabolism
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Humans
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Intercellular Adhesion Molecule-1 / drug effects*
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Pyrrolizidine Alkaloids* / chemistry
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Pyrrolizidine Alkaloids* / isolation & purification
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Pyrrolizidine Alkaloids* / pharmacology
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Streptomyces
Substances
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Benzamides
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Cinnamates
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NP 25301
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NP 25302
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Pyrrolizidine Alkaloids
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Intercellular Adhesion Molecule-1
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bohemamine