Human antibodies from immunized donors are protective against anthrax toxin in vivo

Martha A Wild; Hong Xin; Toshiaki Maruyama; Mary Jean Nolan; Peter M Calveley; John D Malone; Mark R Wallace; Katherine S Bowdish

doi:10.1038/nbt891

Human antibodies from immunized donors are protective against anthrax toxin in vivo

Nat Biotechnol. 2003 Nov;21(11):1305-6. doi: 10.1038/nbt891. Epub 2003 Oct 12.

Authors

Martha A Wild¹, Hong Xin, Toshiaki Maruyama, Mary Jean Nolan, Peter M Calveley, John D Malone, Mark R Wallace, Katherine S Bowdish

Affiliation

¹ Alexion Antibody Technologies, Inc., 3985 Sorrento Valley Blvd., San Diego, California 92121, USA.

PMID: 14555959
DOI: 10.1038/nbt891

Abstract

A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.

MeSH terms

Animals
Antibodies / blood*
Antibodies / immunology*
Antigens, Bacterial*
Bacterial Toxins / antagonists & inhibitors*
Bacterial Toxins / immunology*
Bacterial Toxins / toxicity
Blood Donors
Humans
Immunity / physiology
Immunization / methods
Immunoglobulin Fab Fragments / blood*
Immunoglobulin Fab Fragments / immunology*
Peptide Library
Rats

Substances

Antibodies
Antigens, Bacterial
Bacterial Toxins
Immunoglobulin Fab Fragments
Peptide Library
anthrax toxin