Synthesis of an octamannosyled glycan chain, the key oligosaccharide structure in ER-associated degradation

Carbohydr Res. 2003 Oct 10;338(21):2163-8. doi: 10.1016/s0008-6215(03)00357-4.

Abstract

The high-mannose type decasaccharide (Man(8)GlcNAc(2)), the proposed ligand of ER residing mannosidase-like proteins (MLP), and its monoglycosylated homologue (alpha-Glc(1)Man(8)GlcNAc(2)) were synthesized. The oligosaccharide assembly was performed in a convergent and stereoselective manner, using three oligosaccharide components, a core trisaccharide having a beta-mannoside bond, a liner mannotriose, and a branched mannotetraose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Endoplasmic Reticulum / enzymology
  • Endoplasmic Reticulum / metabolism*
  • Ligands
  • Mannose / chemistry*
  • Mannosidases / metabolism
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / chemistry*
  • Polysaccharides / chemical synthesis*

Substances

  • Ligands
  • Oligosaccharides
  • Polysaccharides
  • Mannosidases
  • Mannose