Correlation of death modes of photosensitized cells with intracellular ATP concentration

FEBS Lett. 2003 Oct 9;553(1-2):167-72. doi: 10.1016/s0014-5793(03)01014-7.

Abstract

The impact of intensity of glycolysis and oxidative phosphorylation on death of photosensitized murine hepatoma MH22 cells in vitro has been investigated. Cells photosensitized with meso-tetra(4-sulfonatophenyl)-porphine localized to lysosomes died mostly by necrosis, and the mode of cell death did not depend on the energy metabolism. Photosensitization with 5-aminolevulinic acid-stimulated endogenous porphyrins localized mainly in mitochondria or 5,10,15,20-tetrakis(m-hydroxyphenyl)-chlorine localized to cell membranes, including mitochondria, led to cell death mostly by apoptosis. In this case, the mode of cell death depended on the medium: under conditions unfavorable to glycolysis the ratio apoptosis/necrosis decreased significantly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Adenosine Triphosphate / pharmacology*
  • Aminolevulinic Acid / pharmacology
  • Animals
  • Apoptosis / drug effects*
  • Apoptosis / radiation effects*
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Glycolysis / drug effects
  • Glycolysis / radiation effects
  • Intracellular Fluid / metabolism*
  • Light
  • Lysosomes / metabolism
  • Membrane Potentials / drug effects
  • Mice
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Necrosis
  • Oxidative Phosphorylation / drug effects
  • Oxidative Phosphorylation / radiation effects
  • Photosensitivity Disorders / chemically induced*
  • Porphyrins / pharmacology
  • Tumor Cells, Cultured

Substances

  • Porphyrins
  • tetraphenylporphine sulfonate
  • Aminolevulinic Acid
  • Adenosine Triphosphate