Abstract
The impact of intensity of glycolysis and oxidative phosphorylation on death of photosensitized murine hepatoma MH22 cells in vitro has been investigated. Cells photosensitized with meso-tetra(4-sulfonatophenyl)-porphine localized to lysosomes died mostly by necrosis, and the mode of cell death did not depend on the energy metabolism. Photosensitization with 5-aminolevulinic acid-stimulated endogenous porphyrins localized mainly in mitochondria or 5,10,15,20-tetrakis(m-hydroxyphenyl)-chlorine localized to cell membranes, including mitochondria, led to cell death mostly by apoptosis. In this case, the mode of cell death depended on the medium: under conditions unfavorable to glycolysis the ratio apoptosis/necrosis decreased significantly.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism*
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Adenosine Triphosphate / pharmacology*
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Aminolevulinic Acid / pharmacology
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Animals
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Apoptosis / drug effects*
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Apoptosis / radiation effects*
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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Glycolysis / drug effects
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Glycolysis / radiation effects
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Intracellular Fluid / metabolism*
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Light
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Lysosomes / metabolism
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Membrane Potentials / drug effects
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Mice
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Mitochondria / drug effects
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Mitochondria / metabolism
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Necrosis
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Oxidative Phosphorylation / drug effects
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Oxidative Phosphorylation / radiation effects
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Photosensitivity Disorders / chemically induced*
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Porphyrins / pharmacology
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Tumor Cells, Cultured
Substances
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Porphyrins
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tetraphenylporphine sulfonate
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Aminolevulinic Acid
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Adenosine Triphosphate