Application of time-resolved diffuse reflectance techniques in studies of reaction intermediates in suspensions of Bacillus subtilis

A B Berinstain; J C Scaiano; C Bohne; P R Malenfant; G D Sprott

doi:10.1111/j.1751-1097.1992.tb02183.x

Application of time-resolved diffuse reflectance techniques in studies of reaction intermediates in suspensions of Bacillus subtilis

Photochem Photobiol. 1992 Oct;56(4):423-6. doi: 10.1111/j.1751-1097.1992.tb02183.x.

Authors

A B Berinstain¹, J C Scaiano, C Bohne, P R Malenfant, G D Sprott

Affiliation

¹ Department of Chemistry, University of Ottawa, Canada.

PMID: 1454871
DOI: 10.1111/j.1751-1097.1992.tb02183.x

Abstract

Short lived reaction intermediates such as triplet states and free radicals can be detected in vivo using laser photolysis techniques with time-resolved diffuse reflectance detection. This novel approach is illustrated for bacterial suspensions of Bacillus subtilis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / metabolism*
Free Radicals / metabolism
Lasers
Photolysis
Spectrophotometry / methods

Substances

Free Radicals