Abstract
Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a chromosomally encoded meta pathway. Phenotypic, chemotaxonomic and genotypic characterization classified strain CLN100 as a member of the species Pseudomonas stutzeri. DNA-DNA hybridizations, 16S rDNA, gyrB, rpoD sequences, and molecular fingerprinting indicate that strain CLN100 is a representative of a new genomovar (genomovar 10) within the species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / isolation & purification
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Base Composition
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Base Sequence
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Biodegradation, Environmental
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DNA Fingerprinting
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DNA Gyrase / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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DNA-Directed RNA Polymerases / genetics
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Genes, Bacterial
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Molecular Sequence Data
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Naphthalenes / metabolism*
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Nucleic Acid Hybridization
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Phylogeny
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Proteome / analysis
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Pseudomonas stutzeri / classification*
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Pseudomonas stutzeri / growth & development
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Pseudomonas stutzeri / isolation & purification
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Pseudomonas stutzeri / metabolism*
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RNA, Ribosomal, 16S / genetics
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Salicylates / metabolism*
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Sigma Factor / genetics
Substances
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Bacterial Proteins
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DNA, Bacterial
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Naphthalenes
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Proteome
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RNA, Ribosomal, 16S
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Salicylates
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Sigma Factor
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RNA polymerase sigma 70
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DNA-Directed RNA Polymerases
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DNA Gyrase