Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro- and methylaromatics: a new genomovar within the species

Syst Appl Microbiol. 2003 Sep;26(3):390-403. doi: 10.1078/072320203322497428.

Abstract

Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a chromosomally encoded meta pathway. Phenotypic, chemotaxonomic and genotypic characterization classified strain CLN100 as a member of the species Pseudomonas stutzeri. DNA-DNA hybridizations, 16S rDNA, gyrB, rpoD sequences, and molecular fingerprinting indicate that strain CLN100 is a representative of a new genomovar (genomovar 10) within the species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / isolation & purification
  • Base Composition
  • Base Sequence
  • Biodegradation, Environmental
  • DNA Fingerprinting
  • DNA Gyrase / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • DNA-Directed RNA Polymerases / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Naphthalenes / metabolism*
  • Nucleic Acid Hybridization
  • Phylogeny
  • Proteome / analysis
  • Pseudomonas stutzeri / classification*
  • Pseudomonas stutzeri / growth & development
  • Pseudomonas stutzeri / isolation & purification
  • Pseudomonas stutzeri / metabolism*
  • RNA, Ribosomal, 16S / genetics
  • Salicylates / metabolism*
  • Sigma Factor / genetics

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Naphthalenes
  • Proteome
  • RNA, Ribosomal, 16S
  • Salicylates
  • Sigma Factor
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases
  • DNA Gyrase