The study was focused on the effects of solid culture and suspension culture on the callus growth and saponin content of callus from the buds of Panax quinquefolius L. The results showed that the growth carves were all "S" shape under two cultural ways. Compared with the solid culture, the whole course of suspension cultrure was advanced five days, and the dry weight of callus was higher than that under solid culture. The content of the saponin, amine acid and starch also. The studies showed that it was possible to produce saponin qinsenosides by large-scale of Panax quinquefolius L.